• 2019-10
  • 2019-11
  • 2020-03
  • 2020-07
  • 2020-08
  • br Inevitably this study has limitations For example the


    Inevitably, this study has limitations. For example, the animal injection method as well as the quantity and frequency of DNT cell and gemcitabine treatments was not based on previously published models.
    In summary, our study demonstrated that pancreatic cancer growth is significantly inhibited by DNT cells both in vivo and in vitro and that the Fas/FasL signaling pathway is involved in the mechanism of DNT cell cytotoxicity. More importantly, this research may provide a preclinical basis for DNT cell therapy for pancreatic cancer.
    Conflicts of interest
    The authors declare that they have no competing interests.
    This present study was funded by the National Natural Science Foundation of China (grant no. 81071985) and the Anhui Province Science and Technology Projects Foundation (grant no. 10080703038).
    [8] Janssen O, Sanzenbacher R, Kabelitz D. Regulation of activation-induced cell death of mature T-lymphocyte populations. Cell Tissue Res 2000;301:85e99. [9] Lundy SK, Lerman SP, Boros DL. Soluble egg antigen-stimulated T helper lymphocyte apoptosis and evidence for cell death mediated by FasL(รพ) T and PPT during murine Schistosoma mansoni infection. Infect Immun 2001;69: 271e80.
    [10] Nagafuchi H, Wakisaka S, Takeba Y, Takeno M, Sakane T, Suzuki N. Aberrant expression of Fas ligand on anti-DNA autoantibody secreting B lymphocytes in patients with systemic lupus erythematosus: "immune privilege"-like state of the autoreactive B cells. Clin Exp Rheumatol 2002;20:625e31.
    [14] Lee J, Minden MD, Chen WC, Streck E, Chen B, Kang H, et al. Allogeneic human double negative T cells as a novel immunotherapy for acute myeloid leukemia and its underlying mechanisms. Clin Canc Res 2018;24:370e82.
    [15] Pitti RM, Marsters SA, Lawrence DA, Roy M, Kischkel FC, Dowd P, et al. Genomic amplification of a decoy receptor for Fas ligand in lung and colon cancer. Nature 1998;396:699e703. [16] Awasthi N, Zhang C, Schwarz AM, Hinz S, Schwarz MA, Schwarz RE. Enhancement of nab-paclitaxel antitumor activity through addition of mul-titargeting antiangiogenic agents in experimental pancreatic cancer. Mol Canc Therapeut; 13: 1032-1043.
    [20] Kabelitz D, Wesch D, He W. Perspectives of gammadelta T cells in tumor immunology. Cancer Res 2007;67:5e8. [21] Lundqvist A, Yokoyama H, Smith A, Berg M, Childs R. Bortezomib treatment and regulatory T-cell depletion enhance the antitumor effects of adoptively infused NK cells. Blood 2009;113:6120e7.
    [22] Bremer E, ten Cate B, Samplonius DF, de Leij LF, Helfrich W. CD7-restricted activation of Fas-mediated apoptosis: a novel therapeutic approach for acute T-cell leukemia. Blood 2006;107:2863e70.  [23] Chang WA, Lin ES, Tsai MJ, Huang MS, Kuo PL. Isolinderalactone inhibits proliferation of A549 human nonsmall cell lung cancer cells by arresting the cell cycle at the G0/G1 phase and inducing a Fas receptor and soluble Fas ligand-mediated apoptotic pathway. Mol Med Rep 2014;9:1653e9.
    [26] Boldin MP, Varfolomeev EE, Pancer Z, Mett IL, Camonis JH, Wallach D. A novel protein that interacts with the death domain of Fas/APO1 contains a sequence motif related to the death domain. J Biol Chem 1995;270:7795e8.
    [27] Chinnaiyan AM, O'Rourke K, Tewari M, Dixit VM. FADD, a novel death domain-containing protein, interacts with the death domain of Fas and ini-tiates apoptosis. Cell 1995;81:505e12. [28] Xu YR, Dong HS, Yang WX. Regulators in the apoptotic pathway during spermatogenesis: killers or guards? Gene 2016;582:97e111. [29] Kimura S, Kawakami T, Kawa Y, Soma Y, Kushimoto T, Nakamura M, et al. Bcl-2 reduced and fas activated by the inhibition of stem cell factor/KIT signaling in murine melanocyte precursors. J Invest Dermatol 2005;124:229e34. [30] Ren X, Zhang Y, Li C, Wang H, Jiang Z, Zhang Z, et al. Enhancement of baicalin by hexamethylene bisacetamide on the induction of apoptosis contributes to simultaneous activation of the intrinsic and extrinsic apoptotic pathways in human leukemia cells. Oncol Rep 2013;30:2071e80.
    [31] Yuan Y, Zhang Y, Zhao S, Chen J, Yang J, Wang T, et al. Cadmium-induced apoptosis in neuronal cells is mediated by Fas/FasL-mediated mitochondrial apoptotic signaling pathway. Sci Rep 2018;8:8837. [32] Cai L, Liu J, Wang Y, Chen H, Ma Y, Wang Y, et al. Enhanced anti-melanoma efficacy of interferon a-2b via overexpression of ING4 by enhanced Fas/ FasL-mediated apoptosis. Oncol Lett 2018;15:9577e83.
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